Research Letter
Multiplex Detection and Genotyping of Point Mutations Involved in Charcot-Marie-Tooth Disease Using a Hairpin Microarray-Based Assay
Table 1
Genes, detected mutations and primers used for multiplex PCR and amplified fragment lengths.
| | Primers | Mutation | Gene | Exon amplified | T m | GC% | Fragment length (bp) | Primer sequence |
| | Multi 1 (F) | V95M | Cx32 | Exon 2 | 67.20 | 45.83 | 574 bp | AGCTTGCTTCATGGCTGGTGTTTT | | Multi 1 (R) | 67.15 | 59.09 | ACTGTGTTGGGGCAGGGGTAGA | | Multi 2 (F) | V113F | P0 | Exon 3 | 67.86 | 52.00 | 285 bp | CTCTCACATGCTTCCCCTCATTCCT | | Multi 2 (R) | T124M | 67.70 | 52.00 | CAAACTGCTTCCCATACCCTTGTCC | | Multi 3 (F) | C42R | PMP22 | Exon 3 | 67.78 | 52.00 | 197 bp | TCCTTCCCCTTTTCCTTCACTCCTC | | Multi 3 (R) | 67.66 | 48.00 | ACAAGCTCATGGAGCACAAAACCAG |
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