The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

Figure 2

(a) Effect of the two substrates (UDP-[¹⁴C]glucose and UDP-ManNAcA) on the activity of teichuronic acid synthetase (TUAS). Open circle: with one substrate 0.5 mM UDP-C¹⁴-Glucose; Closed circle: two substrates by adding 0.5 mM UDP-ManNAcA to the same mix. (b) Analysis of teichuronic acid, the enzymatic product of TUAS, by nondenaturing PAGE stained with Alcian blue and silver. The numbers on the right represent the repeats of the basic TUA disaccharide unit. D is an oligosaccharide of TUA estimated to have 6–10 disaccharide repeats, which is not detectable by PAGE.

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