The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

Table 2

Distribution of TUAS activity in subcellular extract of M. luteus.

Cellular compartment	Specific activity (units*/mg protein)
Control medium	0
Culture supernatant	8.7
Cytoplasm (S-20 fraction)	0.5
Cell wall **	6.2
Cytoplasmic membrane**	19.9

* 1 Unit = 1 nmole [¹⁴C]glucose incorporated in 60 minutes in standard assay.
** Separated by sucrose density gradient centrifugation.