Research Article
Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus
Table 2
Comparison of the binding properties of bcNOS to other bacterial NOS-like and mammalian NOS oxygenase domain proteins.
| Binding constants (M) | bcNOS | bsNOS | deiNOS | iNOSoxy | nNOSoxy |
| imidazole | 181 ± 13 | 384 ± 10 | — | 158 ± 6 | — | L-Arg | 596 ± 161 | 129 ± 2 | 97 ± 10 | 175 ± 4 | 55 ± 4 | L-Arg | 11 ± 3 | 4.8 ± 0.1 | — | 16.1 ± 0.7 | — | NOHA | 1.12 ± 0.01 | — | — | — | — |
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taken from [8]. taken from [11]. iNOSoxy: inducible NOS oxygenase domain. nNOSoxy: neuronal NOS oxygenase domain.
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