Research Article

Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus

Table 2

Comparison of the binding properties of bcNOS to other bacterial NOS-like and mammalian NOS oxygenase domain proteins.

Binding constants ( M)bcNOSbsNOS deiNOS iNOSoxy nNOSoxy

imidazole181 ± 13384 ± 10158 ± 6
L-Arg596 ± 161129 ± 297 ± 10175 ± 455 ± 4
L-Arg11 ± 34.8 ± 0.116.1 ± 0.7
NOHA1.12 ± 0.01

taken from [8]. taken from [11]. iNOSoxy: inducible NOS oxygenase domain. nNOSoxy: neuronal NOS oxygenase domain.