Research Article

Inhaled Anesthetics Promote Albumin Dimerization through Reciprocal Exchange of Subdomains

Figure 4

Anesthetic agents promote albumin dimerization. HSA[A] and BSA[B] were treated with (red) and without (black) agent (sevoflurane [A] and isoflurane [B]) prior to size exclusion chromatography and quantitation of the peaks. Tracings in (a) are smoothed curves that represent averages from four independent experiments. Tracings in (b) are from a representative experiment. Column size and flow rates differed slightly between (a) and (b) with gamma globulin (158‚ÄČkDa) eluting at 13.1 and 19.2 minutes, respectively.
516704.fig.004a
(a)
516704.fig.004b
(b)