Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

Figure 1

Affinity purification of Cephalosporium lectin on asialofetuin-Sepharose 4B column. Crude extract was passed through affinity column, equilibrated in PBS, and the bound lectin was eluted with elution buffer. Fractions of 3.0 ml were collected at a flow rate of 15 ml/hr. Absorbance at 280 nm, Hemagglutinating activity. Inset- SDS-PAGE of affinity purified CSL in 15% gel. The purified lectin (30 𝜇g) is indicated in lane 1 and lane 2 contains standard molecular weight markers. The gel was stained with Coomassie brilliant blue.