Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic
Fungus Causing Mycotic Keratitis
Figure 3
Binding of CSL to human PBMCs and inhibition of binding with competing glycoconjugates. PBMCs were stained with FITC-labeled CSL and subjected to flow cytometric analysis. -axis, FL1-H on a log scale represents the fluorescence intensity of cells stained with FITC labeled CSL. -axis represents cell number. (a) The histoplot shows profiles of the unstained cells (UNS) and cells stained with FITC-labeled CSL (CSL). Profiles of cells stained with FITC-labeled CSL preincubated with different haptens are indicated in (b, c and d).
