Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

Table 1

Purification of CSL from mycelial extract.

Sample	Volume (ml)	Protein (mg)	Sugar (mg)	MCAa (𝜇g)	Specific activityc (units)	Total activityd (units)	Fold purification	Recovery of activity (%)
Original crude extract	41.0	30.053	22.96	1.145	0.087 × 10^4	2.61 × 10^4	—	100
Affinity purified	7.5	0.410	NDb	0.021	4.76 × 10^4	1.95 × 10^4	54.71	74.71

a-Minimum concentration of protein required to agglutinate erythrocytes used.
b-Not detected by phenol-sulfuric acid method.
c-Specific activity: hemagglutinating activity mg⁻¹ protein.
d-Total activity: hemagglutinating activity of lectin in total protein.