AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone
Figure 4
The effect of siRNA knockdown on c-Fos, c-Jun, Runx2, MMP-13, and GAPDH mRNA expression in the IL-1β -induced mesenchymal stromal cells of GCT. GCT stromal cells were transfected by electroporation with corresponding siRNA for 48 h. Treated samples were examined using real-time PCR. The CT method was used to calculate the real-time RT-PCR fold change using RPS18 mRNA as an endogenous control. Three independent experiments were performed. mRNA expression of (a) c-Jun, (b) Runx2, (c) MMP-13, and (d) GAPDH upon treatment with combinations of siRNA. is versus random nonspecific siRNA control. Statistical comparison by analysis of variance with post hoc Tukey’s tests.
