AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone

Table 1

Human primer sequences specially designed for real-time RT-polymerase chain reaction (PCR) amplification.

Gene	Forward/Reverse	Primer sequence	Accession no.	Size of product (bp)	Melting temperature (°C)
c-Fos	F	5^' AGA ATC CGA AGG GAA AGG AA 3^'	NM_005252	150	63.6
	R	5^' CTT CTC CTT CAG CAG GTT GG 3^'			63.8
c-Jun	F	5^' CAG GTG GCA CAG CTT AAA CA 3^'	NM_002228	80	63.8
	R	5^' GTT TGC AAC TGC TGC GTT AG 3^'			63.5
MMP-13	F	5^' CTT CCC AAC CGT ATT GAT GC 3^'	NM_002427	143	64.1
	R	5^' TTT GGA AGA CCC AGT TCA GA 3^'			62.2
Runx2	F	5^' TCT GGC CTT CCA CTC TCA GT 3^'	NM_004348	142	64.0
	R	5^' AAG GTG GCT GGA TAG TGC AT 3^'			63.4
RPS18	F	5^' GAT GGG CGG CGG AAA ATA G 3^'	NM_022551	165	68.4
	R	5^' GCG TGG ATT CTG CAT AAT GGT 3^'			65.8
GAPDH	F	5^' CAT GAG AAG TAT GAC AAC AGC CT 3^'	NM_002046	113	62.0
	R	5^' AGT CCT TCC ACG ATA CCA AAG T 3^'			62.5