In vivo Study of the Histone Chaperone Activity of Nucleolin by FRAP
Figure 3
Nucleolin depletion leads to a faster H2B-eGFP fluorescence recovery kinetics. (a) Example of the fluorescence intensity recovery of H2B-eGFP histone in a nucleolin-depleted cell. Scale bars represent 5 μm. (b) Normalized fluorescence recovery (%) of H2B-eGFP in the nucleoplasm of control-untransfected cells (red, 6 cells) and in cell transfected with siRNA against nucleolin (blue, 2 cells). Each curve represents the fluorescence recovery of a single cell. (c) Average of the normalized fluorescence curves in (b) control cell (red) and nucleolin depleted cells (blue). (d) Comparison of the percentage of fluorescence recovery at 1h30 for control cell (red) and nucleolin-depleted cells (blue). (e) Comparison of the percentage of fluorescence recovery at 2h30 for control cell (red) and nucleolin-depleted cells (blue). (f) Dissociation rate constant values () of H2B-eGFP in control-untransfected (red) and nucleolin-depleted cells (blue).
