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Biochemistry Research International
Volume 2011, Article ID 369484, 7 pages
http://dx.doi.org/10.1155/2011/369484
Review Article

Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Dipartimento di Scienze della Vita e dell'Ambiente, Università di Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy

Received 27 June 2011; Accepted 1 August 2011

Academic Editor: Tau Chuan Ling

Copyright © 2011 Rosaria Medda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods. In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes. The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield. This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources.