Bioseparation of Four Proteins from <i>Euphorbia characias</i> Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td align="left">Step</td><td align="center">Total protein (mg)</td><td align="center">Total activity    (nkat)</td><td align="center">Specific activity    (nkat/mg)</td><td align="center">Yield    (%)</td><td align="center">Purification    (fold)</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td align="left">Acetone powder crude extract</td><td align="center">3500</td><td align="center">418567</td><td align="center">119.6</td><td align="center">100</td><td align="center">1</td></tr><tr><td align="left">Ammonium sulfate fractionation</td><td align="center">534</td><td align="center">165133</td><td align="center">309</td><td align="center">39.4</td><td align="center">2.6</td></tr><tr><td align="left">DEAE–cellulose chromatography</td><td align="center">101</td><td align="center">92700</td><td align="center">917.8</td><td align="center">22</td><td align="center">7.6</td></tr><tr><td align="left">Gel filtration chromatography</td><td align="center">5.9</td><td align="center">58567</td><td align="center">9927</td><td align="center">14</td><td align="center">83</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Purification of <i>Euphorbia</i> latex purple acid phosphatase.

Biochemistry Research International

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Table 3

Table 3: Bioseparation of Four Proteins from <i>Euphorbia characias</i> Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase 

Table 3 | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase 