Mechanism(s) of Toxic Action of Zn2+ and Selenite: A Study on AS-30D Hepatoma Cells and Isolated Mitochondria
Figure 4
Action of cyclosporine A on changes in intracellular ROS formation produced by high Zn2+ and selenite in AS-30D cells. (a) Se: 50 μM; 3 h; (b) Zn: 250 μM; 24 h. The cells were cultivated in RPMI 1640 medium without (control) or with the indicated concentration (in μM) of selenite and Zn2+ for 3 h and 24 h, respectively, in the absence or presence of 1 μM CsA. The overall ROS production was calculated as the geometric mean of total green fluorescence of the oxidation product of DCFH2 and is indicated in the upper right corner of each panel; for other details, see Section 2. A typical experiment out of at least three independent ones for each compound is shown.
