Mechanism(s) of Toxic Action of Zn²⁺ and Selenite: A Study on AS-30D Hepatoma Cells and Isolated Mitochondria

Table 2

Effects of high concentrations of Zn²⁺ and selenite on mitochondrial transmembrane potential of AS-30D cells monitored by flow cytometry after staining the cells with the lipophilic cationic probe JC-1.

Time	50 𝜇M Se	250 𝜇M Zn	500 𝜇M Zn
3 h	67±11 *	55±9 *	46±2 *
24 h	14±3 *	13±1 *	12±4 *

The percentage of cells with red JC-1 fluorescence, reflecting high ΔΨ_mito (expressed in % to the corresponding control), is presented; the percentage of cells with high ΔΨ_mito in untreated control is accepted as 100%. Mean ±𝑆𝐸; 𝑛=3 independent experiments; *𝑃 < 0.05 compared to untreated control.