Mechanism(s) of Toxic Action of Zn²⁺ and Selenite: A Study on AS-30D Hepatoma Cells and Isolated Mitochondria

Table 3

Effects of high concentrations of Zn²⁺ and selenite on the uncoupled respiration of AS-30D cells. The rate of uncoupled respiration (in the presence of CCCP) of control cells is accepted as 100%.

Time	50 𝜇M Se	250 𝜇M Zn	500 𝜇M Zn
3 h	86.4±4.6 *	48.4±7.2 *	28.3±10.6 *
24 h	n.d.	31.0±5.0 *	10.7±0.5 *
48 h	20.8±6.5 *	11.3±1.3 *	1.9±0.3 *

Rate of oxygen uptake is presented in percentage of the maximal (fully uncoupled) respiration of the control cells. Mean ± SE; 𝑛=3 independent experiments; *𝑃 < 0.05 compared to untreated control. For other details, see Section 2; n.d., not determined.