ER Stress and Iron Homeostasis: A New Frontier for the UPR
Figure 2
Regulation of CRT mRNA by iron. Linear regression analysis of the correlation between intracellular iron levels (3 μM ferric nitrilotriacetate (55Fe–NTA) were used as iron source), and CRT mRNA pools in HepG2 cells overexpressing the WT or mutated (C282Y or H63D) HFE variants (HepG2 cells transfected with the empty pcDNA3 vector were used as negative control). Each dot represents the average of one experiment with three replicates. ; . Inner dashed curves represent 95% confidence intervals for the mean value of CRT at any selected CPM value. Outer dashed lines are 95% prediction limits for new observations. Modified from Pinto et al., 2008 [59].
