The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance

Figure 2

The effect of HFD on the LIPA activity measured as FFA release from endogenous TAG. (a) Homogenate; (b) dense lysosomes; (c) light lysosomes. 10% liver homogenate, light or dense lysosomal fraction were incubated 60 min at pH  = 4.5. At the end of incubation, the released FFAs were quantified as described in Section 2. The graph shows the difference between FFA concentration in the sample at the beginning and at the end of the incubation. Open bars = fasted animal; hatched bars = fed animals. ⁺⁺⁺𝑃<0.001 fed versus fasted; ^∗𝑃<0.05 HFD fasted versus SD fasted; ^#𝑃<0.05, ^##𝑃<0.01 HFD fed versus SD fed.

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