Biochemistry Research International

Research Article

The Increased Activity of Liver Lysosomal Lipase in Nonalcoholic Fatty Liver Disease Contributes to the Development of Hepatic Insulin Resistance

Table 1

The effect of HFD on physical and metabolic parameters.
Standard dietHigh-fat diet
FastedFedFastedFed
Body weight (g) 3 3 1 ± 6 . 9 3 7 9 ± 1 0 . 7 x
Epididymal fat pad/b.w. (g/100 g) 0 . 9 ± 0 . 0 7 1 ± 0 . 0 6 1 . 4 ± 0 . 0 7 ∗ 1 . 4 ± 0 . 0 4 #
Glycemia (mmol/L) 5 . 1 ± 0 . 1 7 . 9 ± 0 . 5 + 5 . 9 ± 0 . 2 ∗ 8 . 1 ± 0 . 3 +
Insulinemia (pmol/L) 5 6 ± 1 5 1 3 5 ± 2 1 + 1 2 5 ± 1 0 ∗ 1 2 7 ± 1 8
A U C 0 - 1 8 0 (mmol glucose/L) 1 1 6 8 ± 2 6 . 6 1 3 2 5 ± 3 2 . 9 x
Serum Tg (mmol/L) 0 . 7 ± 0 . 1 1 . 4 ± 0 . 0 8 + 0 . 7 ± 0 . 0 2 1 . 4 ± 0 . 1 +
Serum FFA (mmol/L) 0 . 7 ± 0 . 0 5 0 . 4 ± 0 . 0 2 + 0 . 6 ± 0 . 0 8 0 . 4 5 ± 0 . 0 7 +
ALT (μkat/L) 1 . 2 ± 0 . 1 1 . 2 ± 0 . 1
AST (μkat/L) 4 . 3 ± 0 . 6 3 . 9 ± 0 . 3
β-hydroxybutyrate (μmol/L) 1 . 6 7 ± 0 . 0 5 0 . 0 5 ± 0 . 0 1 + 3 . 2 ± 0 . 2 5 ∗ 0 . 2 8 ± 0 . 0 5 # , +
Data are given as means ± SEM, 𝑛 = 7 . x 𝑃 < 0 . 0 5 HFD versus SD group; + 𝑃 < 0 . 0 5 fasted versus fed animals; ∗ 𝑃 < 0 . 0 5 SD- versus HFD-fasted animals; # 𝑃 < 0 . 0 5 SD- versus HFD-fed animals.