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Biochemistry Research International
Volume 2012 (2012), Article ID 261736, 6 pages
http://dx.doi.org/10.1155/2012/261736
Review Article

Behaviour of Human Erythrocyte Aggregation in Presence of Autologous Lipoproteins

1Instituto de Medicina Molecular, Unidade de Biologia Microvascular e Inflamação, Instituto de Bioquímica Faculdade de Medicina da Universidade de Lisboa. Av Prof. Egas Moniz, 1649-028 Lisboa, Portugal
2Departmento de Cardiologia, Hospital Fernando da Fonseca. IC19, 2720-276 Amadora, Portugal
3Departmento de Medicina I, Faculdade de Medicina da Universidade de Lisboa. Av Prof. Egas Moniz, 1649-028 Lisboa, Portugal
4Instituto de Biopatologia Química Faculdade de Medicina da Universidade de Lisboa. Av Prof. Egas Moniz, 1649-028 Lisboa, Portugal

Received 20 April 2011; Accepted 7 July 2011

Academic Editor: Terry K. Smith

Copyright © 2012 C. Saldanha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this work was to evaluate in vitro the effect of autologous plasma lipoprotein subfractions on erythrocyte tendency to aggregate. Aliquots of human blood samples were enriched or not (control) with their own HDL-C, LDL-C, or VLDL-C fractions obtained from the same batch by density gradient ultracentrifugation. Plasma osmolality and erythrocyte aggregation index (EAI) were determined. Blood aliquots enriched with LDL-C and HDL-C showed significant higher EAI than untreated aliquots, whereas enrichment with VLDL-C does not induce significant EAI changes. For the same range of lipoprotein concentrations expressed as percentage of osmolality variation, the EAI variation was positive and higher in presence of HDL-C than upon enrichment with LDL-C ( ). Particle size, up to LDL diameter values, seems to reinforce erythrocyte tendency to aggregate at the same plasma osmolality (particle number) range of values.