Research Article

Caveolin 3, Flotillin 1 and Influenza Virus Hemagglutinin Reside in Distinct Domains on the Sarcolemma of Skeletal Myofibers

Figure 3

Flotillin 1 is more sparingly soluble in cold Triton X-100 than cav 3. Isolated myofibers were treated with 0, 3, and 5 mM CDX and then extracted with 1% Triton X-100. Soluble material (S) and pellets (Ps) were subjected to SDS/PAGE and western blotting using specific antibodies. Treatment of the myofibers with CDX only slightly increased the solubility of flotillin 1 to the detergent, whereas the solubility of cav 3 remained unaffected.
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