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Biochemistry Research International
Volume 2012, Article ID 541906, 8 pages
Research Article

Association of TCTP with Centrosome and Microtubules

1Department of Developmental Biology & Invertebrate Morphology, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
2CNRS, UMR 6290, Institut de Génétique et Développement de Rennes, Cell Cycle Group, 35043 Rennes, France
3UEB, IFR 140, Faculté de Médecine, Université Rennes 1, 35043 Rennes, France
4Department of Genetics & Evolution, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
5Department of Embryology, Institute of Zoology, University of Warsaw, 02-096 Warszawa, Poland
6Department of Surgery, The Methodist Hospital, Houston, TX 77030, USA
7Immunobiology Laboratory, The Methodist Hospital Research Institute, Houston, TX 77030, USA

Received 16 February 2012; Accepted 6 March 2012

Academic Editor: R. Mark Ghobrial

Copyright © 2012 Mariusz K. Jaglarz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Translationally Controlled Tumour Protein (TCTP) associates with microtubules (MT), however, the details of this association are unknown. Here we analyze the relationship of TCTP with MTs and centrosomes in Xenopus laevis and mammalian cells using immunofluorescence, tagged TCTP expression and immunoelectron microscopy. We show that TCTP associates both with MTs and centrosomes at spindle poles when detected by species-specific antibodies and by Myc-XlTCTP expression in Xenopus and mammalian cells. However, when the antibodies against XlTCTP were used in mammalian cells, TCTP was detected exclusively in the centrosomes. These results suggest that a distinct pool of TCTP may be specific for, and associate with, the centrosomes. Double labelling for TCTP and γ-tubulin with immuno-gold electron microscopy in Xenopus laevis oogonia shows localization of TCTP at the periphery of the γ-tubulin-containing pericentriolar material (PCM) enveloping the centriole. TCTP localizes in the close vicinity of, but not directly on the MTs in Xenopus ovary suggesting that this association requires unidentified linker proteins. Thus, we show for the first time: (1) the association of TCTP with centrosomes, (2) peripheral localization of TCTP in relation to the centriole and the γ-tubulin-containing PCM within the centrosome, and (3) the indirect association of TCTP with MTs.