Figure 4: Comparison of normalized pCa-tension relationships in detergent-skinned fibers reconstituted with cTnC_{WT}, , or . Isometric steady-state tensions elicited by each fiber in various pCa solutions were normalized with its respective value in pCa 4.3 solution. The normalized tensions were plotted against pCa to construct the pCa-tension relationship. The Hill equation was fitted to the normalized pCa-tension relationships to estimate myofilament sensitivity () and the Hill coefficient (). and values are shown in Figures 5(a) and 5(b), respectively. The curves presented here are Hill fits to pCa-tension relationships in cTnC_{WT} (○), (□), and (∆) fibers, respectively. Values are expressed as mean SEM. Number of fibers tested in each group is as follows: .