Sumoylation of Human Translationally Controlled Tumor Protein Is Important for Its Nuclear Transport

Figure 4

Over expression of TCTP conferred resistance to H₂O₂ damage in mammalian cells. Cos1 cells were over expressed with wild type (FL-TCTP) or mutant TCTP (Mut-TCTP) and exposed to 100 μM H₂O₂ for 24 h in culture. Following incubation, cell viability was determined colorimetrically. Cells transfected with vector alone and exposed to the same conditions (O_x-Ctrl) or without exposure to H₂O₂ (Utctrl) served as controls. Results show that mutation of TCTP at the SUMO motif significantly reduced its antioxidant function. *𝑃<0.01 compared to controls.