Differential Expression of Matrix Metalloproteases in Human Fibroblasts with Different Origins
Figure 3
Alteration of MMP expression levels in cardiac, dermal, and pulmonary fibroblasts after treatment with 10 ng/mL TNF- for 24 hours. Different alteration of collagenases (a), stromelysins (b), and MMP9 (c) expression levels after TNF- treatment. MMP9 activity was further determined in the cell culture supernatant of cardiac, dermal, and pulmonary fibroblasts. Representative bands of MMP9 activity in dermal and pulmonary cell culture supernatants with or without TNF- for 72 hours are shown. No MMP9 activity could be detected in cell culture supernatant of cardiac fibroblasts. All mRNA expression levels are shown as absolute expression using the formula .
