Differential Expression of Matrix Metalloproteases in Human Fibroblasts with Different Origins

Table 1

Absolute mRNA expression levels of different MMPs in cardiac, dermal, and pulmonary human fibroblasts. The mRNA expression was normalized to the housekeeping gene CDKN1b using the formula 2^{−Δ𝐶𝑡}. For statistical significance dermal or pulmonary expression was compared to the expression in cardiac fibroblasts using Mann-Whitney U test.

	Absolute mRNA expression level
	(normalized to CDKN1b)
Gene	Cardiac fibroblast	Dermal fibroblast			Pulmonary fibroblast
	Expression	Expression	x-fold to cardiac fibroblast	𝑃 value	Expression	x-fold to cardiac fibroblast	𝑃 value
MMP1	12±3	0.49±0.08	−24	<0.0001	0.67±0.12	−17	<0.0001
MMP2	104±10	111±6	1.1	0.559	88±8	−1.2	0.411
MMP3	0.29±0.08	2.1±0.3	7.0	<0.0001	0.033±0.007	−8.9	0.155
MMP7	0.005±0.001	0.0024±0.0006	−2.0	0.013	0.0005±0.0001	−9.2	<0.0001
MMP8	0.040±0.013	0.0006±0.0002	−63	<0.0001	0.0007±0.0002	−54	<0.0001
MMP9	0.010±0.003	0.028±0.005	2.7	0.003	0.0055±0.0012	−1.9	0.292
MMP14	41±5	15±1	−2.8	<0.0001	26±2	−1.6	0.033