Biochemistry Research International

Review Article

Acetylcholinesterase Biosensors for Electrochemical Detection of Organophosphorus Compounds: A Review

Table 5

OP biosensors based on screen-printed electrodes.
Mode of detectionTransducerEnzyme immobilization methodMinimum detection limitLinearity Substrate/enzyme inhibitorTime of incubation
(min)		Storage stability
(days)		Reference
AmperometricTMOS sol-gel/SPEEncapsulation1.0 × 10−3 μM1.0 and 3.0 × 10−3 μMDichlorvos15NR[68]
AmperometricAl2O3 sol-gel/SPEAdsorption0.01 μM0.1–80 μMDichlorvos155[71]
AmperometricSol-gel/TCNQ/SPEEntrapment1 × 10−2, 
8 × 10−4, and 2 × 10−2 μM		NRCarbaryl, carbofuran, and pirimicard2045[72]
AmperometricSPE/TCNQ/Graphite ElectrodeAdsorption3.0 × 10−6 μM5 × 10−2–0.2 μMChlorpyrifos-ethyl oxon1050[79]
AmperometricCoPC/SPECrosslinking4.9 × 10−4 μM 10-5-1.0 μMCarbofuran15NR[80]
AmperometricPhenylenediamine/
CoPC SPE		Entrapment1 × 10−11, 
1 × 10−10, and 1 × 10−10 μM		1.0 × 10−11 
1.0 × 10−2 μM		Dichlorvos, parathion, and azinphos1092[81]
AmperometricGraphite-epoxy/SPECrosslinking1.0 × 10−4 and 1.0 × 10−5 μMNRParaoxon and carbofuran155[82]
AmperometricGlutaraldehyde vapour/SPECrosslinking0.18 μM0.18–54.00 μMParaoxon10NR[83]
AmperometricPVA-SbQ/SPEEntrapment1.91 × 10−2 μM
1.24 × 10−3 μM		NRParaoxon and chlorpyrifos-ethyl oxon10Nr[36]
AmperometricSWCNTs-CoPC/SPECovalent Bonding0.01 and 6.3 × 10−3 μM0.018–0.181 and 6.36 × 10−3–0.159 μMParaoxon and malaoxon153[84]
AmperometricTCNQ modified-graphiteScreen printing1 ppb0–5 × 10−3 MMethamidophos10NR[85]
AmperometricGold electrodeCrosslinking0.1 mM1–10 mMParaoxon  28[86]
AmperometricCarbon electrodeCovalent Bonding10−10 MNRDichlorvos60NR[87]
AmperometricSPECopolymerisation4 to 7 μg/LNRDicholrvos, methyl-parathion4 NR[88]
Note: NR: not reported.