O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Figure 4

Agarose gel electrophoresis of the O serogroup-specific TMPCR and 16S rDNA PCR profiles using representative V. cholerae gDNAs archived from the clinical specimens. PCR products obtained by both TMPCR (5 μL) and 16S rDNA PCR (5 μL) that were separately performed are confined to the same lane: lanes 1–3, the O1 from stools; lanes 4-5, the O1 from rectal swabs; lanes 6–10, the O139 from stools; and lanes 11–15, the non-O1/non-O139 from stools. In each TMPCR employing the O1, O139, or non-O1/non-O139 gDNA, the reliable reaction represents duplex DNA fragments (588 bp and 364 bp from the O1 or 588 bp and 256 bp from the O139) and a single 588 bp DNA band from the non-O1/non-O139, as compared to 16S rDNA amplicons (663 bp) and 100 bp DNA ladder marker (lane M).