O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Table 5

Agreement of O serogroup-specific TMPCR for detecting and differentiating gDNAs of the O1, O139, and non-O1/non-O139 serogroups archived from clinical samples ( = 148), using serotyping method as reference.

Source	Serogroup^a	TMPCR
		ompW	rfbV	wbf
Stool	O1 ()	65	65	0
	O139 (	15	0	15
	Non-O1/non-O139 ()	28	0	0
	Total	108	65	15
Rectal swab	O1 ()	39	39	0
	O139 ()	0	0	0
	Non-O1/non-O139 ()	1	0	0
	Total	40	39	0

All laboratory settings yielded 100% concordant V. cholerae cultures whether rectal swabs or stools examined using standard serotyping method as described in the text.