O Serogroup-Specific Touchdown-Multiplex Polymerase Chain Reaction for Detection and Identification of Vibrio cholerae O1, O139, and Non-O1/Non-O139

Table 6

Advancement of TMPCR for V. cholera detection using environmental samples.

Source^a	Sample number	Culture method and serotyping			TMPCR
		O1	O139	Non-O1/Non-O139	O1	O139	Non-O1/Non-O139
Water samples	50	0	0	0	0	0	1
Seafood	18	5	0	8	5	0	8

All were collectively obtained during the outbreaks. The individual samples enriched in broth were used in V. cholerae detection and differentiation using the culture method/serotyping and TMPCR.