A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera
Table 1
Purification and percent recovery of BmPI from B. monosperma seeds.
Purification steps
Total volume (mL)
Total protein (mg)
Total trypsin inhibitory unit (TIU)
Specific activity (TIU/mg protein)
Purification factor
Yield (% recovery)
Crude inhibitor extract from seeds (100 gm)
300
2736.82
147744.79
53.98
1.00
100
Heat treatment (heat stable protein) at 60°C; 30 min
257
1935.23
144612.42
74.72
1.38
97.88
F30–65% (NH4)2SO4 saturation fraction
15
512.34
41779.20
81.54
1.51
28.27
Gel filtration (Sephadex G-75) chromatography
2
68.26
16766.70
245.63
4.55
11.34
Trypsin-Sepharose CL-4B affinity chromatography
1
23
11298.98
491.26
9.1
7.68
One inhibition unit is defined as the amount of the inhibitor required to inhibit 50% of trypsin activity, under the trypsin inhibition assay.
