Production and Partial Purification of Alpha Amylase from Bacillus subtilis (MTCC 121) Using Solid State Fermentation

Table 2

Partial purification of alpha amylase.

Sample	Activity; (mg/mL/min)	Protein concentration; (mg/mL)	Specific activity (μmol/min/mg)
Crude	1.56	2	2.27
(0–30%) (NH4)2SO4 cut of crude enzyme	0.208	0.41	1.47
(30–70%) (NH4)2SO4 cut of supernatant obtained from (0–30%) (NH4)2SO4 cut	3.185	1.2	7.72