Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa

Figure 3

Silver stained SDS-polyacrylamide gel electrophoresis demonstrating the purification of 38 kDa RCA binding polypeptide. Lane 1 shows the complex polypeptide pattern present in the detergent soluble fraction of cauda sperm plasma membranes. Lane 2: unbound fraction of DEAE-Sepharose column. Lane 3: 0.1 M NaCl eluted fraction of DEAE-Sepharose column; 38 kDa RCA binding polypeptide was present in this fraction. This fraction was further purified by affinity chromatography on RCA-agarose. Lane 4: unbound fraction of RCA-agarose column. Lane 5: 0.2 M D-galactose eluted fraction of RCA-agarose column. Lectin blot analysis of 0.2 M D-galactose eluted fraction of RCA-agarose column exhibits the presence of the 38 kDa RCA binding polypeptide (lane 6).