Expression and Characterization of the Novel Gene fr47 during Freezing in the Wood Frog, Rana sylvatica

Figure 2

Prediction of SH2 binding motif present in FR47. To elucidate a possible membrane associated function for FR47, DOMPEP was used to identify any possible conserved SH2 binding motifs. (a) Positive identification of the GRB2 SH2 domain phosphotyrosine binding site within the FR47 primary amino acid sequence; “” indicates hydrophobic amino acids. (b) Hypothesized effect of GRB2-SOS signaling on IP3 production and PKC activation. Increased PKC activity is known to increase fr47 production and the presence of a GRB2 binding motif in FR47 could suggest that FR47 may create a feedback mechanism to regulate FR47 expression.

(a)

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