Expression and Characterization of the Novel Gene fr47 during Freezing in the Wood Frog, Rana sylvatica

Figure 3

Relative fr47 transcript expression in response to 24 hr freezing. RT-PCR analysis showing the effects of 24 hr freezing on fr47 mRNA transcript levels in select tissues of the wood frog. (a) Histogram shows mean values (±SEM, -5 independent determinations) for fr47. (b) Representative fr47 and α-tubulin bands used for quantification. Data were analyzed using analysis of variance with a post hoc Student-Newman-Keuls test where the letters “A” (), “B” (), and “C” () represent significant differences between indicated stress and control.

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