Expression and Characterization of the Novel Gene fr47 during Freezing in the Wood Frog, Rana sylvatica

Figure 6

Relative fr47 transcript expression during stages of tadpole development in wood frogs. (a) Histogram shows mean values (±SEM, -5 independent determinations) for fr47. (b) Representative fr47 and α-tubulin bands used for quantification. Data was analyzed using analysis of variance with a post hoc Student-Newman-Keuls test where the letters “A,” “B,” and “C” represent significant differences between Gosner stages 14–20 and later stages, whereas “D” and “E” indicate significant differences between the indicated stage and the one preceding it; “A” and “D” indicate and “B” indicates , while “C” and “E” indicate .

(a)

(b)