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Biochemistry Research International
Volume 2015, Article ID 493879, 12 pages
Research Article

Phytochemical Analysis and Biological Activities of Cola nitida Bark

1Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin
2Laboratoire des Ressources Génétiques et d’Amélioration des Espèces, Département de Génétique et des Biotechnologies, FAST, Université d’Abomey-Calavi, BP 526 Cotonou, Benin
3Université Péléforo Gon Coulibaly de Korhogo UFR des Sciences Biologiques, BP 1328 Korhogo, Cote d'Ivoire
4Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, Attogon, BP 884 Cotonou, Benin
5Laboratoire de Pharmacognosie et des Huiles Essentielles, FSS/UAC, 01 BP 4521 Cotonou, Benin
6Department of Biology and Center for Computational and Integrative Biology, Rutgers University, 315 Penn Street, Camden, NJ 08102, USA
7Laboratoire de Biochimie Alimentaire, Enzymologie, Biotechnologie Industrielle et Bioinformatique, UFR/SVT Université de Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso

Received 20 December 2014; Revised 11 January 2015; Accepted 11 January 2015

Academic Editor: Tzi Bun Ng

Copyright © 2015 Durand Dah-Nouvlessounon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Kola nut is chewed in many West African cultures and is used ceremonially. The aim of this study is to investigate some biological effects of Cola nitida’s bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts of C. nitida were used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolated Staphylococcus strains by disc diffusion method. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent phytochemicals. For the reference and meat isolated strains, the inhibitory diameter zone was from  mm (C. albicans) to  mm (P. vulgaris). The MIC ranged from 0.312 mg/mL to 5.000 mg/mL and the MBC from 0.625 mg/mL to >20 mg/mL. The highest antifungal activity was observed with F. verticillioides and the lowest one with P. citrinum. The two extracts have an excellent reducing free radical activity. The killing effect of A. salina larvae was perceptible at 1.04 mg/mL. The purified extracts of Cola nitida’s bark can be used to hold meat products and also like phytomedicine.