Bp-13 PLA₂: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom

Figure 1

RP-HPLC chromatography of Bothrops pauloensis venom on μ-Bondapak C 18 column (0.78 cm × 30 cm; Waters 991-PDA system Waters). A sample of 20 mg from venom was eluted with solvent B (acetonitrile, 0–66%) at 25°C. The elution profile was monitored at 280 nm. The main fractions obtained are identified as Bp-1–Bp-18.