Research Article
Bp-13 PLA2: Purification and Neuromuscular Activity of a New Asp49 Toxin Isolated from Bothrops pauloensis Snake Venom
Figure 5
Kinetic analysis of Bp-13 PLA2 activity. (a) Influence of calcium ion on PLA2 activity; (b) effect of substrate concentration on the kinetics of Bp-13 PLA2; (c) Lineweaver-Burk (double-reciprocal) plot of Bp-13 PLA2; (d) optimal pH for PLA2 activity; (e) optimal temperature for PLA2 activity; (f) influence of ions (10 mM each) on PLA2 activity in the absence or presence of 1 mM and 10 mM Ca2+. The results are the mean ± SEM of five experiments. when compared with control values. ANOVA and followed by Mann-Whitney test for comparison between two groups.
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