Fabrication and Optimization of ChE/ChO/HRP-AuNPs/c-MWCNTs Based Silver Electrode for Determining Total Cholesterol in Serum

Table 6

Comparison of the present method with previously reported biosensor for total cholesterol determination.

Transducer	Method of enzyme immobilization	Working potential	Response time	Detection limit	Linearity	Storage stability	Reference
Laponite clay nanoparticles-pol((12-pyrrol-1-dodecyl)triethylammonium tetrafluoroborate)/Pt disk electrode	ChO, ChE enzyme Entrapment	0.53 V versus Ag/AgCl	50 sec	20 M	—	20 days	[39]
Screen printed graphite electrode	ChO, ChE, HRP, K4Fe(CN)6; Physical adsorption	−0.2 V versus Ag/AgCl	—	2.81 mM	2.81–13 mM	—	[40]
Polydiaminonaphthalene/Pt disk	ChO, ChE; Entrapment	0.7 V versus Ag/AgCl	15 sec	97 M	Up to 0.8 mM	—	[41]
MWCN/screen printed carbon electrode	ChO, ChE, HRP, K4Fe(CN)6; Physical adsorption	0.3 V versus Ag/AgCl	180 sec	100 mg/dL	100–400 mg/dL	2 months	[27]
3-Aminopropyl-modified controlled-pore glass(APCEG)/rotating disk	ChO, ChE, HRP; Covalent cross-linking via Glutaraldehyde	−0.15 V versus Ag/AgCl with TBC as mediator	—	11.9 nM	1.2 M–1 mM	25 days	[42]
PANI/ITO	ChO, ChE; Covalent cross-linking via Glutaraldehyde	0.5 V versus Ag/AgCl	40 sec	50 mg/dL	50–500 mg/dL	6 weeks	[43]
HRP incorporated carbon paste	ChO, ChE; Covalent cross-linking on PVC beaker	−0.5 V versus Ag/AgCl	20 sec	2.5 mg/dL	50–550 mg/dL	100 days	[38]
Nanoporous Au networks directly grown on a titanium substrate	ChO, ChE, HRP; Physical adsorption; Chitosan used as glue	Cyclic voltammetry	—	0.5 mg/dL	0.97–7.8 mM	60 days	[44]
ZnO–CuO composite matrix grown onto ITO coated corning glass	ChO, ChE; Physical adsorption	Cyclic voltammetry	5 sec	0.5 mM	0.5–12 mM	—	[45]
c-MWCNT/AuNP	ChO, ChE, HRP; Covalent cross-linking via c-MWCNT	0.27 V versus Ag/AgCl	20 sec	0.5 mg/dL	0.5–300 mg/dL	60 days	This work