Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner

Table 4

Viral and cellular genes expression levels in response to H1N1 influenza virus of A549 and MDCK cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for caspases 3, 8, and 9, TNFα, IκBα, BAD, MxA, and -actin and GPDH genes.

H1N1	IκBα		BAD		TNF		Caspase 9		Caspase 8		Caspase 3		MXA		-actin		GPDH
	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0	MOI 0.1	MOI 1.0
MDCK
16 hpi	18.9	20	21.6	21.8	22	22	20	20	22.6	23	22	22	16.5	16.9	17.2	17.2	22.2	22.2
24 hpi	21	21	23.8	24.2	24	24.9	25.4	24.6	25.2	24.6	26	26	20	20.8	17.6	17.6	23.6	23.6
36 hpi	20	19	21.8	22.2	22.6	22.4	23	23.6	23	23	24	24.4	18.4	18.6	18	18	23.2	23.2
48 hpi	18.9	18.6	21.75	21.9	22.9	22.88	21.3	21.6	22.9	23.3	23	23.9	18.0	21.7	18.4	18.4	23	23
A549
16 hpi	19.6	18.7	16.82	17.1	20.7	20.88	21.6	21.2	19.4	19.8	18	17	18.2	29.4	17	16.9	20.7	20.66
24 hpi	23	23.8	19.4	19.6	21.89	22.25	26.7	26.8	22	23	20	20	21	21.3	17.2	17.2	21	21
36 hpi	22	21.98	18.3	18.7	21.2	21.3	24.0	24.2	21.8	21.9	19.2	19.8	20.4	20.6	17.8	17.8	21	21.0
48 hpi	20.45	19.2	17.66	18.2	20.9	20.9	21.81	21.8	20.8	20.8	17.6	18.2	19.2	19.8	17	17.0	21	21.0