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Biochemistry Research International
Volume 2016, Article ID 3136043, 6 pages
http://dx.doi.org/10.1155/2016/3136043
Research Article

Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence

1School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, India
2Department of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, India

Received 27 November 2015; Revised 1 April 2016; Accepted 11 April 2016

Academic Editor: Tzi Bun Ng

Copyright © 2016 Neha Gupta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.