Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
Effect of melatonin, H2O2, and melatonin plus H2O2 on (a) mRNA expression and (b) enzyme activity of MnSOD in PBMCs from healthy subjects. PBMCs were treated as follows: untreated (only with 0.1% DMSO for 12 h); melatonin (1 mM) for 12 h; H2O2 (250 μM) for 2 h; and melatonin plus H2O2 group receiving H2O2 (250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM). All untreated cells were treated with 0.1% of DMSO as a control. Data are expressed as mean ± SEM.