Research Article

Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?

Figure 3

Effect of melatonin, H2O2, and melatonin plus H2O2 on (a) mRNA expression and (b) enzyme activity of MnSOD in PBMCs from healthy subjects. PBMCs were treated as follows: untreated (only with 0.1% DMSO for 12 h); melatonin (1 mM) for 12 h; H2O2 (250 μM) for 2 h; and melatonin plus H2O2 group receiving H2O2 (250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM). All untreated cells were treated with 0.1% of DMSO as a control. Data are expressed as mean ± SEM.