Halophilic Bacteria of Lunsu Produce an Array of Industrially Important Enzymes with Salt Tolerant Activity

Figure 1

Screening and characterization of halophilic bacterial isolates for amylase activity. (a) Equal number of cells of indicated halophilic bacterial isolates (SS1, SS2, SS3, and SS5 and SS8) were spotted on Luria Bertani (LB) agar medium supplemented with 1% starch and 1 M NaCl. The plates were incubated at 30°C for 24 h and flooded with Gram’s iodine to visualize zone of clearance due to starch digestion by amylase. Effect of NaCl, pH, and temperature on amylase activity of halophilic isolates: the specific amylase activity (U/mg) of the bacterial isolates Halobacillus trueperi SS1 and SS3 was plotted against the different concentrations of sodium chloride (NaCl) (b), varying pH (c), and temperature (d) as indicated. In each panel, the data represent an average of three independent experiments with standard deviation.

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