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Figure 3: Screening and characterization of halophilic bacterial isolates for lipase production. (a) Equal number of cells of halophilic bacterial isolates (SS1, SS2, SS3 and SS4, and SS5 and SS8) was spotted on LB agar medium supplemented with 1% Tween-80 and 1 M NaCl. The plates were incubated at 30°C for 24 h to visualize lipase activity. Effect of NaCl, pH, and temperature on lipase activity of halophilic isolates: the specific lipase activity (U/mg) of the bacterial isolate Shewanella algae SS2 was plotted against the different concentrations of sodium chloride (NaCl) (b), varying pH (c), and temperature (d) as indicated. In each panel, the data represent an average of three independent experiments with standard deviation.