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Biochemistry Research International
Volume 2016, Article ID 9519527, 10 pages
http://dx.doi.org/10.1155/2016/9519527
Research Article

Production of Laccase by Cochliobolus sp. Isolated from Plastic Dumped Soils and Their Ability to Degrade Low Molecular Weight PVC

1Department of Virology and DST-PURSE Centre, Sri Venkateswara University, Tirupati 517502, India
2Department of Bionanotechnology, Gachon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do 461 701, Republic of Korea

Received 27 November 2015; Accepted 4 April 2016

Academic Editor: Robert J. Linhardt

Copyright © 2016 Tirupati Sumathi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

One of the utmost man-made problems faced today has been the ever-increasing plastic waste filling the world. It accounts for an estimated 20–30% (by volume) of municipal solid waste in landfill sites worldwide. Research on plastic biodegradation has been steadily growing over the past four decades. Several fungi have been identified that produce enzymes capable of plastic degradation in various laboratory conditions. This paper presents a study that determined the ability of fungi to degrade low molecular weight polyvinyl chloride (PVC) by the enzyme laccase. We have isolated a fungal species, Cochliobolus sp., from plastic dumped soils and they were cultured on Czapek Dox Agar slants at 30°C. The effectiveness of this fungal species on the degradation of commercial low molecular weight polyvinyl chloride (PVC) was studied under laboratory conditions. Significant differences were observed from the FTIR, GC-MS, and SEM results in between control and Cochliobolus sp. treated PVC.