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Biochemistry Research International
Volume 2016 (2016), Article ID 9781216, 9 pages
Research Article

Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F.2.1.4. Obtained by Solid-State and Submerged Cultivation

1Laboratório de Bioquímica de Proteínas, Departamento de Química e Ciências Ambientais, Universidade Estadual Paulista (UNESP), Rua Cristovão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil
2Laboratório de Bioquímica e Microbiologia Aplicadas, Departamento de Biologia, Universidade Estadual Paulista (UNESP), Rua Cristovão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil

Received 16 November 2015; Revised 3 March 2016; Accepted 12 April 2016

Academic Editor: Gary A. Lorigan

Copyright © 2016 Vanessa de Cássia Teixeira da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This work reports endoglucanase and beta-glucosidase production by the thermophilic fungus Myceliophthora heterothallica in solid-state (SSC) and submerged (SmC) cultivation. Wheat bran and sugarcane bagasse were used for SSC and cardboard for SmC. Highest endoglucanase production in SSC occurred after 192 hours: 1,170.6 ± 0.8 U/g, and in SmC after 168 hours: 2,642 ± 561 U/g. The endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their optimal pH and temperature. The number of endoglucanases was also different: six isoforms in SSC and ten in SmC. Endoglucanase activity remained above 50% after incubation between pH 3.0 and 9.0 for 24 h for both cultivation systems. The effect of several chemicals displayed variation between SSC and SmC isoenzymes. Manganese activated the enzymes from SmC but inhibited those from SSC. For β-glucosidases, maximum production on SmC was 244 ± 48 U/g after 168 hours using cardboard as carbon source. In SSC maximum production reached 10.9 ± 0.3 U/g after 240 h with 1 : 1 wheat bran and sugarcane bagasse. Manganese exerted a significant activation on SSC β-glucosidases, and glucose inhibited the enzymes from both cultivation systems. FeCl3 exerted the strongest inhibition for endoglucanases and β-glucosidases.