Figure 1: Evaluation of the inhibitory activity of salicylaldehyde-hydrazine hydrate Schiff base (1a) against S. aureus in vitro. Various concentrations of compound 1a were added to 96-well microculture plates containing the S. aureus strain ATCC 25923 at concentration of 105 CFU/mL in Luria Broth. The absorbance of every well at 450 nm was assayed in an ELISA plate reader after shaking on a vibrating platform at 37°C for 8 h. The inhibition ratio (%) was determined as reported in Materials and Methods. Values are mean ± standard deviation of three independent experiments.