Table 1: The comparison of lipid peroxidation inhibition percentages and ferrous ion (Fe2+) chelating activities of reference antioxidants and some phenolic and flavonoid compounds (10 μg/mL for chelating activity and 20 μg/mL for lipid peroxidation inhibition).

AntioxidantFe2+ ions chelating activity with ferrozine reagentFe2+ ions chelating activity with bipyridyl reagentTotal antioxidant activity
IC50 (µg/mL)% activityIC50 (µg/mL)% activity% inhibition

BHA32.4727.3442.0324.4696.39
BHT30.0734.8931.9828.8663.63
α-Tocopherol25.7338.4112.6672.539.10
Trolox49.4421.3518.5630.8693.57
EDTA7.3295.80
Malvin18.5452.2114.3465.7395.16
ID-818.3354.168.8088.0697.98
Pelargonin25.7239.5814.3051.4693.93
Silychristin36.7825.7824.8314.2095.45
Callistephin34.5122.7320.8033.0697.58
Oenin28.9522.0026.4716.4096.75
Arachidonoyl Dopamine20.3750.6511.0873.8651.14
3,4-Dihydroxy-5-methoxybenzoic acid26.9336.3252.3792.0017.23
2,4,6-Trihydroxy benzaldehyde32.1632.1617.9348.0028.24

The IC50 values were calculated by means of metal chelating and total antioxidant activity graphs from values measured at different concentrations (10–30 µg/mL) of reference antioxidants and the phenolic and flavonoid compounds.