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Biochemistry Research International
Volume 2017 (2017), Article ID 9478958, 9 pages
https://doi.org/10.1155/2017/9478958
Research Article

The Protective Effect of Naringenin-Oxime on Cisplatin-Induced Toxicity in Rats

1Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey
2Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey
3Department of Urology, Faculty of Medicine, Harran University, Sanliurfa, Turkey
4Department of Chemistry, Faculty of Science and Literature, Harran University, Sanliurfa, Turkey

Correspondence should be addressed to Ismail Koyuncu; moc.liamg@1ucnuyokliamsi

Received 12 May 2017; Accepted 19 July 2017; Published 28 August 2017

Academic Editor: Tzi Bun Ng

Copyright © 2017 Ismail Koyuncu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study is to examine the protective effect of naringenin-oxime (NOX) on cisplatin-induced major organ toxicity and DNA damage in rats. Thirty-five male Wistar albino rats were equally split into five groups as follows: control (i.p., 0.1 ml of saline), Cis administration (i.p., 7 mg/kg b.w.), NOX treatment (i.p., 20 mg/kg b.w., daily for ten days), Cis + NOX20, and Cis + NOX40 combination (i.p., 20 and 40 mg/kg b.w., daily for ten days). Serum and peripheral blood mononuclear leukocytes (PBMC) were obtained from blood. Malondialdehyde, glutathione, total antioxidant and oxidant status, and catalase were measured in serum, liver, and kidney, and oxidative stress index was calculated. In parallel, paraoxonase and arylesterase activities were tested in liver and serum. We used 8-OHdOG as a marker for DNA damage in serum via ELISA and in PMBC via comet assay. Treatment with Cis elevated the levels of serum biochemical parameters, oxidative stress, and DNA damage. Pretreatments of NOX restored biochemical and oxidative stress parameters in serum, renal, and liver tissues () and reduced 8-OHdG level, a finding further supported by comet assay in PBMC. Observations of the present study support the fact that treatment with NOX prevents Cis-induced hepatotoxicity, nephrotoxicity, and genotoxicity by restoring antioxidant system.